The seahorse xfe24 extracellular flux analyzer from seahorse bioscience studies the two major energy producing pathways of the cell. Xf analyzers perform compound addition and mixing, labelfree analytical detection, and automatic measurement of ocr. Compact and easy to use, the xfp analyzer is designed to perform standard xf assays, and is ideal for pairwise comparisons. This new, lower cost instrument will enable many more labs access to seahorse s unique technology. Seahorse bioscience xf96 extracellular flux analyzer product. Based analysis of cellular respiration in caenorhabditis elegans anthony l. Seahorse assays enable quantification of cellular bioenergetics by measuring the two energy yielding pathways, mitochondrial respiration and glycolysis, in a sensitive and convenient microplate format. Seahorse bioscience omnify software case study seahorse bioscience xfp extracellular flux analyzer proven xf technology, accessible for every lab. The seahorse extracellular flux xf24 analyzer provides a comprehensive assessment of the relative state of glycolytic and aerobic metabolism in. The measurement of cellular bioenergetics on live cells enables timeresolved analysis and testing of multiple conditions per assay well. The xf96 extracellular flux analyzer was developed to provide higher throughput analysis for customers desiring to study mitochondrial function and dysfunction, obesity, diabetes, cancer, ageing disorders, cardiac disease and drug toxicity. Metabolic profiling of healthy and cancerous tissues in 2d.
Seahorse xf analyzers measure oxygen consumption rate ocr and extracellular acidification rate ecar of live cells in a multiwell plate, interrogating key cellular functions such as mitochondrial respiration and glycolysis. The seahorse xfe24 extracellular flux analyzer from seahorse bioscience studies the two major energy producing pathways of the cell mitochondrial respiration and glycolysis simultaneously, in a 24well microplate format in realtime. Seahorse bioscience sells instruments and consumables. Xfe extracellular flux analyzers simultaneously interrogate the two major energy producing pathways of the cell mitochondrial respiration and glycolysis in a microplate, in realtime. Advances in the quantification of mitochondrial function in.
View and download seahorse xf96 installation and operation manual online. The seahorse extracellular flux xf24 analyzer provides a comprehensive assessment of the relative state of glycolytic and aerobic metabolism in live cells making this method instrumental in. Its novel 8well miniplate format is ideal for use in pairwise comparison assays, as well as for use with primary cells and other precious samples. Seahorse xfe96 extracellular flux analyzer dartlab. Seahorse xfe24 extracellular flux analyzerbased analysis of. Seahorse xf96 installation and operation manual pdf. A global leader in the design and manufacture of innovative life science.
Seahorse xfe96 extracellular flux analyzer the seahorse enables quantitation of metabolomics of live cells in real time with stimulation with up to 4 drugs. Seahorse bioscience, the world leader in metabolic analyzers and xf stress test kits for measuring cell metabolism, introduces the xfp extracellular flux analyzer an affordable extension to the xf product platform. Briefly, cells were plated into xf24 v7 or xf96 v3 polystyrene cell culture plates seahorse bioscience, north billerica. Easily create assay protocols and analyze data with seahorse wave software. The seahorse bioscience xfe analyzer measures the rate of change of dissolved. Dec 14, 2012 the seahorse xf24 extracellular flux analyzer hereafter referred to as the seahorse xf24 analyzer allows nondestructive, noninvasive, and sensitive analysis of the bioenergetic state of live intact cells in realtime providing invaluable assessments of mitochondrial function wu et al. The xfp extracellular flux analyzer is built on innovative and proven xf technology for live cellbased assays of energy metabolism. Box of seahorse xf96 polystyrene cell culture microplates pn 101085004. Seahorse technology is based on measuring the rate of extracellular fluxes of analytes in the media surrounding cells. Measurement of mitochondrial respiration and glycolysis allows energetic phenotyping of cells, in order to assess functional changes. Seahorse xf analyzers measure oxygen consumption rate ocr and extracellular acidification. Measuring energy pathways with the seahorse xf analyzer. The seahorse extracellular flux xfe analysers provide a convenient multiwell plate. The xf extracellular flux analyzer is the bestselling instrument.
The new seahorse xfp analyser has been built on the basis of the proven xf. Seahorse bioscience xfe instrument overview and intended use the seahorse bioscience xfe analyzer measures the rate of change of dissolved oxygen and ph in the media immediately surrounding living cells cultured in a microplate. Seahorse bioscience xf96 extracellular flux analyzer w xf. Windowscompatible desktop analysis software wave for plotting, reporting. Changes in the extracellular media are caused by the consumption or production of analytes by the cells. Livecell metabolic assay platform for life science research. The first published seahorse data was reported in a 2006 nature article. Nov 10, 2017 high throughput metabolic profiling using, e. Seahorse bioscience xf96 extracellular flux analyzer product details the xf96 extracellular flux analyzer is a fullyintegrated instrument that simultaneously measures the two major energy yielding pathways aerobic respiration and glycolysis in a convenient, microplate format. Xfe wave software, designed for the xfe extracellular flux analyzer. Xfp analyzer overview the seahorse bioscience xfp analyzer measures the rate of change of dissolved oxygen and ph in the media immediately surrounding living cells cultured in a miniplate. Comprehensive method for culturing embryonic dorsal root. The seahorse xfp analyzer is a compact and easytouse bench top instrument for analyzing energy metabolism in live cells. Consumables include flux paks, microplates, and stress kits.
The xf24 extracellular flux analyzer is a 24 well tissue culture microplate system that measures oxygen consumption rate ocr and extracellular acidification rate ecar of living cells simultaneously in real time. The xf palmitatebsa fao reagent is a solution absorbed by cells. Technologies seahorse bioscience is a leader in life sciences, diagnostics, and applied chemical markets. Seahorse xf e 96 is the worlds most advanced cell metabolic analyzer and employs fiber optics based. Analysis of lymphocyte mitochondrial function has been facilitated by introduction of 96well format extracellular flux xf96 analyzers, but the technology. Seahorse bioscience announces the xf96 extracellular flux analyzer for cellular bioenergetics published. Furthermore, changes in mitochondrial function may regulate lymphocyte function in inflammatory diseases like type 2 diabetes.
Ocr and ecar measurements were performed using the xf24 or xf96 extracellular flux analyzer seahorse bioscience, north billerica, ma as described. Seahorse xf24 extracellular flux analyzer department of. Tool kit seahorse bioscience xfe96 extracellular flux. Rapid analysis of glycolytic and oxidative substrate flux of. Metabolic analysis rare cells, seahorse xf hs mini analyzer agilent. Seahorse xfe analyzers from agilent cell analysis selectscience. Seahorse xf cell energy phenotype test kit receives top 10.
Sunnybrook research institute sri is home to the worlds most advanced metabolic data analyzer. The xf96 extracellular flux analyzer seahorse bioscience is a noninvasive, labelfree, highthroughput instrument that measures the metabolic activity of living cells by simultaneously monitoring mitochondrial respiration and glycolysis. Frontiers comprehensive method for culturing embryonic. The seahorse bioscience xf e 96 extracellular flux analyzer simultaneously measures oxygen consumption rate and extracellular acidification rate of living cells cultured in disposable 96well microplates.
Quick overview preowned and in working condition computer included with software flux analyzer part no 100900101 pre station part no 101200100 manual included especificacoes. Extracellular flux analysis to monitor glycolytic rates. The xf96 cell culture plate seahorse bioscience was coated with 10 l of celltak bd biosciences which were prepared a working solution in. Seahorse bioscience liaises with volpi for xfe extracellular flux analyzer.
This instrument measures oxygen consumption rate ocr and extracellular acidification rate ecar of living cells simultaneously in a 24 well format. Seahorse xfe96 analyzer measures ocr and ecar of live cells in a 96well plate format. Xfp extracellular flux analyzer from agilent cell analysis. Seahorse xf96 installation and operation manual pdf download. Forms a microchamber and calculates rates of extracellular flux in minutes. Seahorse wave desktop software is the assay design and data analysis software for all agilent seahorse xf analyzers. The seahorse extracellular flux xf analyzer seahorse bioscience is a powerful tool for measuring glycolysis and oxidative phosphorylation through oxygen consumption simultaneously in the same cells.
Seahorse bioscience introduces xfe the worlds most. The seahorse bioscience xf96 system includes xf96 analyzer. This product eliminates the need for radioactivity in fatty acid oxidation. Designed by scientists for scientists, seahorse bioscience products include the seahorse extracellular flux xf analyzer, and xf stress test kits to make cellular bioenergetic studies simple, efficient, and userfriendly. Here, using the seahorse xf e 24 extracellular flux analyzer seahorse bioscience, massachusetts, usa we describe how to measure the fundamental parameters of the electron transport chain etc. Seahorse bioscience liaises with volpi for xfe extracellular. Seahorse bioscience announces the xf96 extracellular flux. Used with the seahorse xfp extracellular flux analyzer, the xf cell energy phenotype test kit measures the utilization of each major energy pathway of a cell mitochondrial respiration and. Seahorse bioscience xf96 extracellular flux analyzer wins.
117 1598 953 1631 973 1514 1374 1322 453 645 1102 1108 1389 1475 871 5 859 539 1517 55 1428 1282 1482 1011 699 1474 734 1177 183 559